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中文名称 | BTB/POZ结构域蛋白19抗体 |
别 名 | BB115071; BTB/POZ domain-containing protein 19; BTBD19; BTBDJ_HUMAN; RP23-59L24.3. |
研究领域 | 细胞生物 免疫学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Mouse, Rat, (predicted: Human, Pig, ) |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 32kDa |
细胞定位 | 细胞核 细胞浆 细胞外基质 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human BTBD19:101-200/291 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
PubMed | PubMed |
产品介绍 | |
产品图片 | Sample: Cerebellum (Mouse) Lysate at 40 ug Primary: Anti- BTBD19 (bs-8401R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 30 kD Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD19) Polyclonal Antibody, Unconjugated (bs-8401R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD19) Polyclonal Antibody, Unconjugated (bs-8401R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD19) Polyclonal Antibody, Unconjugated (bs-8401R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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