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环指蛋白193抗体

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中文名称 环指蛋白193抗体
别    名 bcl-2 ARE RNA binding protein; mex 3 homolog D (C. elegans); MEX 3D; mex3; OK/SW-cl.4; ring finger (C3HC4 type) and KH domain containing 1; Ring finger and KH domain containing 1; RKHD1; RNF193; tino.  
研究领域 细胞生物  免疫学  结合蛋白  表观遗传学  环指蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 65kDa
细胞定位 细胞核 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MEX3D/RNF193:201-300/651 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Mex3d is a ubiquitously expressed protein. Due to alternative splicing events truncating the N-terminus, a variant form of Mex3d exists and is known as TINO.

Function:
RNA binding protein, may be involved in post-transcriptional regulatory mechanisms.

Subcellular Location:
Cytoplasm Nucleus

Tissue Specificity:
Ubiquitously expressed in all the cell lines and tissues tested.

Post-translational modifications:
Phosphorylated

Similarity:
Contains 2 KH domains.
Contains 1 RING-type zinc finger.

SWISS:
Q86XN8

Gene ID:
399664

Database links:

Entrez Gene: 399664 Human

Omim: 611009 Human

SwissProt: Q86XN8 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample: U251 Cell (Human) Lysate at 40 ug
Primary: Anti-MEX3D/RNF193 (bs-18822R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (bs-18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (bs-18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF193) Polyclonal Antibody, Unconjugated (bs-18822R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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