XPA Rabbit Polyclonal Antibody （KO Validated）

英文名称：XPA Rabbit Polyclonal Antibody （KO Validated）
产品类别：抗体
产品编号：K5336
产品应用：WB, IF
性状：Liquid
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid

规格

价格库存

50μl

￥ 880 200

立即订购

订购热线：021-34661276

收藏此商品

我要询价

商品详情
售后服务
相关文献

来源	用途	交叉反应性	理论分子量	实际分子量
Rabbit	WB, IF	H, M	31kDa	37kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ChIP	ChIP-seq
1:500-1:2000	-	1:50-1:100	-	-	-	-	-

抗体详细信息如下::

About this Antibody
Name	XPA Rabbit Polyclonal Antibody (KO Validated)
Category	Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype	IgG
Purification method	Affinity purification
Positive samples	HeLa, Jurkat, K-562, Mouse liver, Mouse kidney
Cellular location	Nucleus
Customer validation	-
About the Immunogen
Immunogen	Recombinant fusion protein of human XPA (NP_000371.1).
Sequence	MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM
Gene ID	7507
Swiss Prot	P23025
Synonyms	XPA; XP1; XPAC; DNA repair protein complementing XP-A cells
Category	DNA Damage and Repair
Background	Nucleotide excision repair (NER) is a process by which cells identify and repair DNA lesions that result from chemical and radiation exposure. The DNA binding protein XPA is an essential part of a pre-incision complex that forms at sites of damage, and is necessary for the initiation of nucleotide excision repair. XPA is one of eight NER proteins (XPA-G, XPV) encoded by genes that are defective in cases of xeroderma pigmentosum, a disorder characterized by sensitivity to sunlight, predisposition to exposed tissue cancers, and neurological defects in some patients. Activation of XPA follows phosphorylation at Ser196 and results in increased NER activity. Phosphorylation of XPA at Ser196 is induced by UV exposure in an ATR-dependant fashion and promotes nuclear accumulation of XPA. Research studies suggest that XPA may be a direct substrate of the serine/threonine kinase ATR and that NER activity may be negatively regulated through dephosphorylation of Ser196 by the phosphatase WIP1.

包装清单：

产品编号	产品名称	包装
AF5336	XPA Rabbit Polyclonal Antibody (KO Validated)	50μl
AZ050	Western一抗稀释液	50ml
—	说明书	1份

保存条件：
XPA Rabbit Polyclonal Antibody (KO Validated) -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

代表性图片：


Fig. 1. Western blot analysis of extracts of various cell lines, using XPA antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231).	Fig. 2. Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody at 1:1000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time:1s.

Fig. 3. Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.	Fig. 4. Immunofluorescence analysis of A549 cells using XPA antibody.