BID Rabbit Polyclonal Antibody

英文名称：BID Rabbit Polyclonal Antibody
产品类别：抗体
产品编号：K6306
产品应用：WB, IF
性状：Liquid
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid

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价格库存

50μl

￥ 880 200

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来源	用途	交叉反应性	理论分子量	实际分子量
Rabbit	WB, IF	H, M, R	11/14/21/26kDa	22kDa

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：

WB	IP	IF	IHC	ICC	FC	ChIP	ChIP-seq
1:500-1:2000	-	1:50-1:200	-	-	-	-	-

抗体详细信息如下::

About this Antibody
Name	BID Rabbit Polyclonal Antibody
Category	Rabbit Polyclonal Antibody (pAb); Primary antibody
Isotype	IgG
Purification method	Affinity purification
Positive samples	HL-60
Cellular location	Cytoplasm, Mitochondrion membrane, Mitochondrion membrane
Customer validation	-
About the Immunogen
Immunogen	Recombinant fusion protein of human BID (NP_001187.1).
Sequence	MDCEVNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGYDELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPGLVNGLALQLRNTSRSEEDRNRDLATA
Gene ID	637
Swiss Prot	P55957
Synonyms	BID; FP497; BH3-interacting domain death agonist
Category	Regulation of Apoptosis; Inhibition of Apoptosis; Death Receptor Signaling; Mitochondrial Control of Apoptosis
Background	This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.

包装清单：

产品编号	产品名称	包装
AF6306	BID Rabbit Polyclonal Antibody	50μl
AZ050	Western一抗稀释液	50ml
—	说明书	1份

保存条件：
BID Rabbit Polyclonal Antibody -20ºC保存，Western一抗稀释液-20ºC或4ºC保存，一年有效。Western一抗稀释液优先推荐4ºC保存，长期不使用可以考虑-20ºC保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

代表性图片：


Fig. 1. Western blot analysis of extracts of HL-60 cells, using BID Antibody at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A).	Fig. 2. Immunofluorescence analysis of C6 cells using BID antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Fig. 3. Immunofluorescence analysis of NIH/3T3 cells using BID antibody at dilution of 1:100. Blue: DAPI for nuclear staining.	Fig. 4. Immunofluorescence analysis of U2OS cells using BID antibody at dilution of 1:100. Blue: DAPI for nuclear staining.