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泛素蛋白单克隆抗体

英文名称：Ubiquitin
产品类别：抗体
产品编号：33307M
产品应用：WB IHC-P IHC-F ICC IF
性状：Liquid
纯化方法：affinity purified by Protein A
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid

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英文名称Ubiquitin

中文名称泛素蛋白单克隆抗体

别名FLJ25987; MGC8385; RPS27A; RPS 27A; UBA52; UBA 52; UBA80; UBA 80; UBB; UBC; UB; UBCEP1; UBCEP2; UBCEP 1; UBCEP 2; UBCEP-1; UBCEP-2; Ubiquitin; ubiquitin B; UBC_HUMAN; UBB_HUMAN; RS27A_HUMAN; HMG20; Ubiquitin carboxyl extension protein 80; Ubiquitin; Polyubiquitin-C; Polyubiquitin-B.

研究领域肿瘤细胞生物神经生物学细胞凋亡泛素

抗体来源Mouse

克隆类型Monoclonal

克隆号8A10

交叉反应Human, Mouse, Rat,

产品应用WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分子量8.5kDa

细胞定位细胞核细胞浆

性状Liquid

浓度1mg/ml

免疫原KLH conjugated synthetic peptide derived from human Ubiquitin:

亚型IgG

纯化方法affinity purified by Protein G

储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Function:

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Subcellular Location:

Cytoplasm. Nucleus.

Similarity:

Belongs to the ubiquitin family.

Contains 9 ubiquitin-like domains.

SWISS:

P0CG48

Gene ID:

7314

Database links:

Entrez Gene: 281370 Cow

Entrez Gene: 100049794 Horse

Entrez Gene: 7314 Human

Entrez Gene: 22187 Mouse

Entrez Gene: 192255 Rat

Omim: 191339 Human

SwissProt: P0CG53 Cow

SwissProt: Q8MKD1 Horse

SwissProt: P0CG47 Human

SwissProt: P0CG48 Human

SwissProt: P62988 Human

SwissProt: P0CG49 Mouse

SwissProt: P62991 Mouse

SwissProt: P0CG51 Rat

SwissProt: P62989 Rat

Unigene: 356190 Human

Unigene: 520348 Human

Unigene: 282093 Mouse

Unigene: 371592 Mouse

Unigene: 110618 Rat

Unigene: 1253 Rat

Unigene: 3761 Rat

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Monoclonal Antibody, Unconjugated (bsm-33307M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.