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英文名称HSP90 beta
中文名称热休克蛋白90β/HSP90 β 单克隆抗体
别 名HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Heat shock protein HSP 90-beta; Heat shock 84 kDa.
研究领域肿瘤 免疫学 信号转导 转录调节因子
抗体来源Mouse
克隆类型Monoclonal
克 隆 号2A2
交叉反应Human, Mouse, Rat,
产品应用WB=1:500-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量84kDa
细胞定位细胞浆
性 状Liquid
浓 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HSP90 beta:
亚 型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].
Function:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Subunit:
Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.
Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications:
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).
Similarity:
Belongs to the heat shock protein 90 family.
SWISS:
P08238
Gene ID:
3326
Database links:
Entrez Gene: 3326 Human
Entrez Gene: 15516 Mouse
Entrez Gene: 301252 Rat
Omim: 140572 Human
SwissProt: P08238 Human
SwissProt: P11499 Mouse
SwissProt: P34058 Rat
Unigene: 509736 Human
Unigene: 2180 Mouse
Unigene: 98667 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
HSP是细胞受应激原刺激后诱导产生的一组应激蛋白，与肿瘤发生、增殖及分化有关，HSP90β能够抑制细胞凋亡与分化。
| 产品图片 |  Sample: Hela KO HSP90 beta(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD  Sample: K562 (Human) Lysate at 30 ug Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD  Sample: Hela (Human) Lysate at 30 ug Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 84 kD Observed band size: 84 kD  Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 4: K562 (Human) Cell Lysate at 30 ug Lane 5: HepG2 (Human) Cell Lysate at 30 ug Lane 6: U2os (Human) Cell Lysate at 30 ug Lane 7: A549 (Human) Cell Lysate at 30 ug Lane 8: HL60 (Human) Cell Lysate at 30 ug Lane 9: Jurkat (Human) Cell Lysate at 30 ug Lane 10: Testis (Mouse) Lysate at 40 ug Lane 11: Testis (Rat) Lysate at 40 ug Lane 12: Cerebrum (Mouse) Lysate at 40 ug Lane 13: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 86 kD  Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining. |
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