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WILL-1(人B细胞淋巴瘤)

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I. General information 
Synonyms: WILL-1
Background: established in 2007 from the bone marrow mononuclear cells of an 82-year-old Japanese man with diffuse large B-cell lymphoma in the leukemic phase
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell lymphoma
Gender: N/A 
Morphology: small round or teardrop-shaped cells growing singly or in clumps in suspension;
Growth Mode: N/A 
Doubling Time: ca. 30-50 hours; but 50-70 hours after recovery from liquid nitrogen
DNA Profile: N/A
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Culture Medium: RPMI-1640+20%FBS
We strongly suggest to purchase the complete medium from
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +,CD80 +, CD138 +, HLA-DR +;
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by species PCR
Cytogenetics: human bimodal hypodiploid/hyperdiploid karyotype; 45(41-46)<2n>X, -Y, add(1)(q24), der(2)t(1;2)(q12;q3?7)inv(1)(q2?4q4?1), i(4q), der(6)t(6;8)(q27;q24), t(8;14)(q24;q32), der(17)t(X;17)(q23;p13), i(21q); sdl with idem, 57(50-58)<2n>X, -Y, +5, +6, +7, +8, +9, +11, +13, +der(14), +20, +21; carries t(8;14) effecting IGH/MYC rearrangement confirmed by FISH; non-IGH-MYC rearrangement present in der(6); resembles published karyotype
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
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