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U-2940(人B细胞淋巴瘤)

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I. General information 
Synonyms: U-2940
Background: established in 1991 from the pleural effusion of a 18-year-old woman with diffuse large B-cell lymphoma (DLBCL) after being diagnosed earlier with Hodgkin lymphoma and treated with chemotherapy; cells were described in the literature to bear microsatellite instability
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell lymphoma
Gender: N/A 
Morphology: small round cells growing singly and in clusters in suspension; 
Growth Mode: N/A 
Doubling Time: ca. 50 hours
DNA Profile: N/A
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Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium from 
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, cm/cylambda -;
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by species PCR
Cytogenetics: human hypodiploid karyotype with 8% polyploidy; 45(43-45)X, -X, del(3)(p14p21), del(6)(q13q15), der(7)t(2;7)(q22;p22), dup(12)(q13q22), der(14)t(X;14)(q12;p11), t(16;16)(p12;p13), del(17)(p13); carries homozygous microdeletion of SOCS1 locus; resembles published karyotype
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
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