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SVCT人SV40转化乳腺上皮细胞

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Synonyms: SVCT人SV40转化乳腺上皮细胞
Background: SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.
Species: human (Homo sapiens)
Tissue: breast
Disease: Human breast epithelium, SV40 transformed
Gender: N/A
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS
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Culture Medium: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
   
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