肥大细胞羧肽酶A3抗体

英文名称：CPA3
产品类别：抗体
产品编号：12338R
产品应用：WB ELISA IHC-P IHC-F ICC IF
性状：Liquid
纯化方法：affinity purified by Protein A
保质期：1年
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid

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100ul

￥ 1900 200

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200ul

￥ 2800 200

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英文名称 CPA3

中文名称 肥大细胞羧肽酶A3抗体

别名 carboxypeptidase A; Carboxypeptidase A3; carboxypeptidase A3 mast cell; CBPA3_HUMAN; Cpa3; Mast cell carboxypeptidase A; mast cell carboxypeptidase A3; MC CPA; MC-CPA.

研究领域细胞生物发育生物学信号转导干细胞

抗体来源 Rabbit

克隆类型 Polyclonal

交叉反应 Rat, (predicted: Human, Mouse, )

产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分子量 36kDa

细胞定位细胞浆

性状 Liquid

浓度 1mg/ml

免疫原 KLH conjugated synthetic peptide derived from Human CPA3/MC-CPA:101-200/417

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMed PubMed

产品介绍 Carboxypeptidase A (CPA) is a pancreatic exopeptidase which hydrolyses the peptide bond adjacent to the C-terminal end in polypeptide chains. Mast cell carboxypeptidase A (MC-CPA), a part of the peptidase M14 family, is a highly conserved metalloprotease localized to the secretory granules, along with trytases and chymases. MC-CPA is stored as an active enzyme in the granule and is released, along with other inflammatory mediators, upon mast cell degranulation. MC-CPA mirrors pancreatic carboxypeptidase A in cleaving COOH-terminal aromatic and aliphatic amino acid residues. The optimum pH of MC-CPA is between neutral and basic, depending upon the substrate. The MC-CPA gene, CPA3, resides on chromosome 3 and contains 11 exons.

Subcellular Location:

Cytoplasmic vesicle, secretory vesicle. Secretory granules.

Similarity:

Belongs to the peptidase M14 family.

SWISS:

P15088

Gene ID:

1359

Database links:

Entrez Gene: 1359 HumanOmim: 114851 HumanSwissProt: P15088 HumanUnigene: 646 Human

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CPA3) Polyclonal Antibody, Unconjugated (bs-12338R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.