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Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Amino Acid: 200-500 of human Eg5
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 ?g antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
- AB_2130964 (BioLegend Cat. No. 627801) AB_2130963 (BioLegend Cat. No. 627802)
Antigen Details
- Structure
- Kinesin-like protein family, BimC subfamily, kinesin motor catalytic, coiled-coil, Smc domains; 119 kD
- Distribution
-
Centrosomes, spindle microtubules, intracellular bridge
- Function
- Motor protein required for establishing bipolar spindle
- Interaction
- Thyroid hormone receptor in presence of thyroid hormone, Cdc2
- Modification
- Phosphorylation
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
1. Lee J, et al. 1995. Mol. Endocrinol. 9:243.
2. Blangy A, et al. 1995. Cell 83:1159.
3. Whitehead C, et al. 1998. J. Cell Sci. 111:2551.
4. DeBonis S, et al. 2003. Biochemistry. 42:338. - Regulation
- Phosphorylation on Thr927 by Cdc2 allows association with spindle apparatus
- Gene ID
- 3832 View all products for this Gene ID
- UniProt
- View information about Eg5 on UniProt.org
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