试验前请仔细阅读说明书,本产品仅供科研实验使用。
1. 所购产品均质量保证!
2. 试剂盒方面提供技术指导,Elisa试剂盒还可以提供代检测服务。
3.本公司出售的任何产品均保质保量,除以下情况,客户可联系我公司申请退换货售后服务
以下情况不予办理退换货:
1、已超过受理期限的产品,如过保质期。
2、由于客户自身操作原因导致实验失败。
3、由于客户没有仔细确认要订购的指标,导致自己的指标定错。
4、产品已使用(质量问题除外)。
PG - Quality tested
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq?-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 ?g per million cells in 100 ?L volume. Refer to the corresponding TotalSeq? protocol for specific staining instructions.
Additional reported application (for the relevant formats) includes: blocking the binding of HIV-1 to Langerhans cells1.
TotalSeq? reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library.
Langerhans cells, dermal CD103-positive dendritic cells, and splenic CD8-positive dendritic cells
1. Valladeau J, et al. 2000. Immunity 12:71.
2. Mc Dermott R, et al. 2002. Mol. Biol. Cell. 13:317.
3. Mizumoto N, et al. 2004. J. Clin. Invest. 113:701.
4. Witte LD, et al. 2007. Nat. Med. 13:367.
试验前请仔细阅读说明书,本产品仅供科研实验使用。
1. 所购产品均质量保证!
2. 试剂盒方面提供技术指导,Elisa试剂盒还可以提供代检测服务。
3.本公司出售的任何产品均保质保量,除以下情况,客户可联系我公司申请退换货售后服务
以下情况不予办理退换货:
1、已超过受理期限的产品,如过保质期。
2、由于客户自身操作原因导致实验失败。
3、由于客户没有仔细确认要订购的指标,导致自己的指标定错。
4、产品已使用(质量问题除外)。
暂无数据
扫码咨询微信客服
扫码咨询微信客服