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Purified anti-β-Amyloid, 1-40 Antibody (Prev

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Do not store antibody diluted below 50 ?g/mL in the absence of protein (i.e.: add 2% bovine serum albumin). The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
DOT Blot, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 11A50-B10 is reactive to the C-terminus of beta amyloid and is specific for the isoform ending at the 40th amino acid.

Application References

(PubMed link indicates BioLegend citation)
  1. Watts JC, et al. 2014. Proc. Natl. Sci. USA 28:10323. (IHC-P) PubMed
  2. Diomede L, et al. 2014. Neurobiol. Dis. 62:521. (Dot Plot) PubMed
  3. Watanabe H, et al. 2012. J Neurosci. 15:5085. (ELISA) PubMed
  4. Portelius E, et al. 2006. J. Proteome Res. 4:1010. (IP)
  5. Vingtdeux V, et al. 2011. FASEB J. 25:219. (ELISA) PubMed
  6. Ohmi K, et al. 2011. PLoS One 11:e27461. (IHC-Other) PubMed
Product Citations
  1. Vingtdeux V, et al. 2011. FASEB J. 25:219-231. PubMed
  2. Watts J, et al. 2014. Proc Natl Acad Sci U S A. 111:10323-10328. PubMed
  3. Lisa Dolfe et al. 2018. Journal of Alzheimer's disease reports. 2(1):27-39 . PubMed
  4. Diomede L, et al. 2014. Neurobiol Dis. 62:521-532. PubMed
  5. Watanabe H, et al. 2012. J Neurosci. 32:5085-5096. PubMed
  6. Voth S, et al. 2020. FASEB J. 9156:34. PubMed
  7. Shi H, et al. 2020. Acta Neuropathol. 139:813. PubMed
  8. Bourassa P, et al. 2020. Neuropathol Appl Neurobiol. 46:458. PubMed
  9. Plowey ED, et al. 2022. Acta Neuropathol. 144:143. PubMed
  10. Lee CYD et al. 2018. Neuron. 97(5):1032-1048 . PubMed
  11. Kim K, et al. 2020. Nat Commun. 0.540972222. PubMed
  12. Shi H, et al. 2020. Acta Neuropathol Commun. 0.473611111. PubMed
  13. Ohmi K, et al. 2011. PLoS One. 6:e27461. PubMed
  14. Parker H, et al. 2020. EMBO Mol Med. 12:e11185. PubMed
  15. Ruiz–Riquelme A, et al. 2018. Acta Neuropathol Commun. 6:26. PubMed
  16. Carreras I, et al. 2019. Sci Rep. 9:10972. PubMed
RRID
AB_2734555 (BioLegend Cat. No. 805409) AB_2564680 (BioLegend Cat. No. 805401) AB_2564681 (BioLegend Cat. No. 805402) AB_2564679 (BioLegend Cat. No. 805403)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-40 on UniProt.org
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