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重组人骨形态发生蛋白2(活性的, Tag free)

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英文名称 Recombinant human BMP-2 protein (Active, HEK293)
中文名称 重组人骨形态发生蛋白-2 (活性的, Tag free)
别    名 rhBMP-2; Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A; BMP-2; BMP2_HUMAN; Recombinant human BMP-2 protein (Active, HEK293)  人骨形态发生蛋白-2重组蛋白; 重组人骨形态发生蛋白-2; 人重组骨形态发生蛋白-2; 重组人BMP2;
检测分子量 30-38 kDa
性    状 Lyophilized powder
浓    度 >0.5 mg/ml
物    种 Human
序    列 283-396/396
纯    度 >95% as determined by SDS-PAGE
纯化方法 AC
内毒素 <0.5 EU/μg as determined by LAL.
表达系统 HEK293 cell
活性 Yes
标签 Tag free
缓 冲 液 Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
保存条件 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍

Bone morphogenetic protein 2 (BMP-2) is a member of transforming growth factor β family that includes more than 20 structurally related bone growth factors. BMP-2 is expressed abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. BMP-2 plays an important role in inducing bone and cartilage formation. Mature human BMP-2 is usually a homodimer consisting of two monomers of 114 amino acids. Dimerization is facilitated by a disulfide bridge formed between the monomer, which contains three intrachain disulfide bridges arranged in a cystine knot motif.

This recombinant human BMP-2 is produced by human cells.(Tag free)

Biological activity:
The activity was measured by its ability to induce alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line).

Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
SWISS:
P12643
Gene ID:
650
 

 

 

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The purity of the protein is greater than 95% as determined by non reduced and reduced SDS-PAGE
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