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Pacific Blue™ anti-human CD15 (SSEA-1) Antib

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
Concentration
test sizes: lot-specific; ?g size: 0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 ?l per 106 cells in 100 ?l volume.
For ?g size, the suggested use of this reagent for immunofluorescent staining is ≤1.0 ?g per 106 cells in 100 ?l volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Silvin A, et al. 2020. Cell. 182:1401. PubMed
  2. Girard P, et al. 2022. Front Bioeng Biotechnol. 10:895735. PubMed
  3. Leach SM, et al. 2020. Cell Rep. 33:108337. PubMed
  4. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  5. K?llgaard T, et al. 2015. PLoS One. 10: 0131934. PubMed
  6. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  7. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  8. Iversen T, et al. 2014. Clin Cancer Res. 20:221. PubMed
RRID
AB_2105361 (BioLegend Cat. No. 323021) AB_2105359 (BioLegend Cat. No. 323024) AB_2105360 (BioLegend Cat. No. 323022)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org
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