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Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- GST fused to C-terminal fragment of Lamin A (amino acid residues 422-664)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
WL4G10 does not recognize mouse Lamin A or C.
WL4G10 failed to immunoprecipitate Lamin A or C from HeLa lysates.
During PD testing for ICC applications, WL4G10 staining of PFA-fixed HeLa cells was compatible with both Methanol and Triton X-100 permeabilization methods; observed staining was slightly better with Triton X-100 permabilization.- RRID
- AB_2810655 (BioLegend Cat. No. 600001) AB_2810656 (BioLegend Cat. No. 600002)
Antigen Details
- Structure
- Lamin A is a 664 amino acid protein with a predicted molecular weight of 74 kD. Lamin C is a 572 amino acid protein with a predicted molecular weight of 65 kD.
- Distribution
-
Ubiquitously expressed/Nucleoplasmic side of inner nuclear membrane
- Function
- Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers, Western Blot Controls
- Molecular Family
- Nuclear Markers, Organelle Markers
- Gene ID
- 4000 View all products for this Gene ID
- UniProt
- View information about Lamin A/C on UniProt.org
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