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Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Baboon, Chimpanzee, Cotton-topped Tamarin
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections5. WM15 does not recognize formalin-fixed or paraffin-embedded tissue sections5. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Saiki I, et al. 1993. Int J Cancer. 54:137. (Block)
- Rosenzwajg M, et al. 2000. Blood 95:453. (Block)
- Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed
- Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC)
- Product Citations
-
- RRID
- AB_10900061 (BioLegend Cat. No. 301711) AB_10900260 (BioLegend Cat. No. 301712)
Antigen Details
- Structure
- Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
- Distribution
-
Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset
- Function
- Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine
- Ligand/Receptor
- Coronavirus receptor
- Cell Type
- Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Shipp M, et al. 1993. Blood 82:1052.
2. Larsen S, et al. 1996. J. Exp. Med. 184:183. - Gene ID
- 290 View all products for this Gene ID
- UniProt
- View information about CD13 on UniProt.org
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