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Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human myeloid leukaemia cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy5 to PE/Cyanine5. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine5 products. Please contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
- Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
- Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
- Product Citations
-
- RRID
- AB_314350 (BioLegend Cat. No. 303406)
Antigen Details
- Structure
- Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
- Distribution
-
Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
- Function
- Adhesion and lectin activity
- Ligand/Receptor
- Sugar chains containing sialic acid
- Cell Type
- B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
-
1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005. - Gene ID
- 945 View all products for this Gene ID
- UniProt
- View information about CD33 on UniProt.org
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