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APC anti-human CD3 Antibody

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Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF? purified antibody (Endotoxin < 0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin < 0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ,　et al. 2020.　Proc Natl Acad Sci USA. 117:33455-33465. (SB)　PubMed
  17. Radtke AJ,　et al. 2022.　Nat Protoc. 17:378-401. (SB)　PubMed
Product Citations
  1. Crawford MP, et al. 2020. Proc Natl Acad Sci U S A. 117:19408. PubMed
  2. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  3. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  4. Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed
  5. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  6. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  7. Gutzeit C, et al. 2014. J Immunol. 192:5852. PubMed
  8. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  9. Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed
  10. Ogishi M, et al. 2021. Nat Med. 27:1646. PubMed
  11. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  12. Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed
  13. Paris O, et al. 2022. Front Immunol. 13:975910. PubMed
  14. Lichtenegger FS, et al. 2018. Front Immunol. 9:385. PubMed
  15. Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
  16. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  17. Levring TB, et al. 2019. Sci Rep. 9:16725. PubMed
  18. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  19. Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed
  20. Helmink BA, et al. 2020. Nature. 577:549. PubMed
  21. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  22. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  23. Huang GL, et al. 2020. PLoS One. 15:e0241421. PubMed
  24. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  25. Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed
  26. Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed
  27. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  28. Meraviglia S, et al. 2017. Oncoimmunology. 6:e1347742. PubMed
  29. Fujii S, et al. 2019. Stem Cells Dev. 28:28. PubMed
  30. Hannon E, et al. 2021. PLoS Genet. e1009443:17. PubMed
  31. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  32. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  33. Kim JR, et al. 2005. BMC Immunol. 2005,6:3. PubMed
  34. Ling X, et al. 2022. STAR Protoc. 3:101321. PubMed
  35. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  36. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  37. Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed
  38. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  39. Barresi V, et al. 2020. J Clin Med. 9:00. PubMed
  40. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  41. Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
  42. Morton B, et al. 2021. Nat Commun. 12:3554. PubMed
  43. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  44. Singh KS, et al. 2021. Nature. 589:597. PubMed
  45. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  46. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  47. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  48. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  49. Headland S, et al. 2014. Sci Rep. 4:5237. PubMed
  50. Giron LB, et al. 2020. EBioMedicine. 73.94791667. PubMed
  51. Siegers G, et al. 2011. PLoS One. 6:e16700. PubMed
  52. Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
RRID
AB_314065 (BioLegend Cat. No. 300411) AB_314066 (BioLegend Cat. No. 300412) AB_2564151 (BioLegend Cat. No. 300458) AB_2562045 (BioLegend Cat. No. 300439)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
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