APC anti-human CD31 Antibody

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy², immunohistochemical staining of acetone-fixed frozen tissue sections⁸,　blocking of platelet aggregation³, and spatial biology (IBEX)^11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
11. Radtke AJ,　et al. 2020.　Proc Natl Acad Sci USA. 117:33455-33465. (SB)　PubMed
12. Radtke AJ,　et al. 2022.　Nat Protoc. 17:378-401. (SB)　PubMed

1. Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed
2. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
3. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
4. Cortesi EE, et al. 2021. Cells. 10:. PubMed
5. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
6. Jung S, et al. 2015. J Rheumatol. 42:264. PubMed
7. Wicki A, et al. 2012. Clin Cancer Res. 1.065277778. PubMed
8. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
9. Junghof J, et al. 2022. NPJ Regen Med. 7:14. PubMed
10. Watanabe Y, et al. 2015. Mol Ther Nucleic Acids. 4: e253. PubMed
11. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
12. Ye W, et al. 2022. Regen Ther. 21:477. PubMed
13. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
14. Xu H, et al. 2022. Cell Death Dis. 13:478. PubMed
15. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
16. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
17. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
18. de Sousa BM, et al. 2021. NPJ Regen Med. 6:80. PubMed
19. Miki K, et al. 2021. Nat Commun. 12:3596. PubMed
20. Takeda A, et al. 2020. Immunity. 51(3):561-572. PubMed
21. Singh K, et al. 2017. Mol Ther. 25:2689. PubMed
22. Chu PH, et al. 2020. Chem Res Toxicol. 751:33. PubMed
23. Bharat A, et al. 2020. Science Translational Medicine. 12(574):. PubMed
24. Takeda A, et al. 2020. STAR Protoc. 1:100021. PubMed
25. Antonova LV, et al. 2019. Polymers (Basel). 11:1. PubMed
26. Salumets A, et al. 2022. Aging Cell. 21:e13607. PubMed
27. Wilson SB, et al. 2022. Genome Med. 14:19. PubMed
28. Liu Z, et al. 2018. Cancer Med. 10.1002/cam4.1584. PubMed

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

1. DeLisser H, et al. 1994. Immunol. Today 15:490.
2. Newman P, 1997. J. Clin. Invest. 99:3.
3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.