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当前位置：首页 > 抗体蛋白 > 抗体> FITC anti-human CD31 Antibody

FITC anti-human CD31 Antibody

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Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8,　blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ,　et al. 2020.　Proc Natl Acad Sci USA. 117:33455-33465. (SB)　PubMed
  12. Radtke AJ,　et al. 2022.　Nat Protoc. 17:378-401. (SB)　PubMed
Product Citations
  1. Vincent V, et al. 2020. Biotechniques. 68:325. PubMed
  2. Zhou Y, et al. 2020. Cancer Cell. 38(6):818-828.e5. PubMed
  3. Mulay A, et al. 2021. Cell Reports. 35(5):109055. PubMed
  4. Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed
  5. Liu X, et al. 2020. Exp Ther Med. 1.258333333. PubMed
  6. Luzuriaga J, et al. 2019. Front Physiol. 10:347. PubMed
  7. MacFadyen JR, et al. 2005. FEBS Lett. 2005. PubMed
  8. Nishino K, et al. 2021. Sci Rep. 11:8406. PubMed
  9. Marquez-Curtis LA, et al. 2022. PLoS One. 17:e0263005. PubMed
  10. Dyring-Andersen B, et al. 2020. Nat Commun. 4.338194444. PubMed
  11. Wang D, et al. 2019. Cell Res. 29:832. PubMed
  12. Downes DJ, et al. 2021. Nat Genet. 53:1606. PubMed
  13. Marjanovic ND, et al. 2020. Cancer Cell. 38(2):229-246.e13. PubMed
  14. Hamauchi S, et al. 2016. PLoS One. 11: 0163561. PubMed
  15. Koide M, et al. 2018. Tohoku J Exp Med. 244:15:00. PubMed
  16. Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed
  17. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. PubMed
  18. Bieniasz–Krzywiec P, et al. 2020. Cell Metabolism. 30(5):917-936. PubMed
  19. Zhai X, et al. 2018. Oncol Lett. 15:1893. PubMed
  20. King D, et al. 2019. Sci Rep. 9:4157. PubMed
  21. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  22. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  23. Nodomi S, et al. 2016. Oncogene. 10.1038/onc.2016.72. PubMed
  24. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  25. Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed
  26. Kamali K, et al. 2021. Front Immunol. 12:647900. PubMed
  27. Sarveswaran K, et al. 2016. Sci Rep. 6:21885. PubMed
  28. Laird C, et al. 2017. Xenotransplantation. 10.1111/xen.12294. PubMed
  29. Li L, et al. 2020. Cell Res. . PubMed
  30. Njock MS, et al. 2022. J Extracell Vesicles. 11:e12228. PubMed
  31. Liang H, et al. 2018. Oncogene. 15:1961. PubMed
  32. Escribano J, et al. 2019. PLoS Comput Biol. 15:e1006395. PubMed
  33. Luzuriaga J, et al. 2020. Biomedicines. 8:00. PubMed
  34. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  35. Yoon YJ, et al. 2022. Nat Commun. 13:3291. PubMed
  36. Wu SZ, et al. 2020. EMBO J. 39:e104063. PubMed
  37. Chen G, et al. 2015. J Endod. 41: 1091-1099. PubMed
  38. Hajal C, et al. 2022. Nat Protoc. 17:95. PubMed
RRID
AB_314329 (BioLegend Cat. No. 303103) AB_314330 (BioLegend Cat. No. 303104)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
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