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Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.
- Application References
(PubMed link indicates BioLegend citation) -
- Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC)
- Springer TA. 1994. Cell 76:301. (IHC)
- Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP, Block)
- Devine L, et al. 1996. Immunol. 88:456. (Block)
- Ohta Y, et al. 1998. Transplantation 65:1094. (Block)
- Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)
- RRID
- AB_2749874 (BioLegend Cat. No. 201902)
Antigen Details
- Structure
- 160-170 kD glycoprotein
- Distribution
-
Leukocytes including lymphocytes, monocytes/macrophages, and granulocytes.
- Function
- Mediates intercellular adhesion and cellular activation.
- Interaction
- CD18
- Ligand/Receptor
- CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3)
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855. - Gene ID
- 308995 View all products for this Gene ID
- UniProt
- View information about CD11a on UniProt.org