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Product Details
- Verified Reactivity
- Rat, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- M12.4.1.C3 cells transduced with rat CD1d
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F, IP, WB - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of CD1d-mediated antigen presentation to type I and II NKT cells1, immunoprecipitation1, Western Blotting1, and immunohistochemical staining of frozen tissue sections1. Clones WTH-1 and WTH-2 recognize different epitopes of CD1d.
- Application References
(PubMed link indicates BioLegend citation) -
- Monzon-Casanova E, et al. 2010. PLoS ONE 5:e13089.
- RRID
- AB_11147755 (BioLegend Cat. No. 205802)
Antigen Details
- Structure
- Member of the MHC family, type I transmembrane protein, associates with β2-microglobulin
- Distribution
-
Dendritic cells, monocytes, macrophages, B cells, T cells, thymocytes, intestinal epithelial cells
- Function
- Presents glycolipid antigens to iNKT cells
- Ligand/Receptor
- Vα14i TCR
- Cell Type
- B cells, Dendritic cells, Epithelial cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens, TCRs
- Antigen References
-
1. Arrenberg P, et al. 2010. P. Natl. Acad. Sci. USA. 107:10984.
2. Mattarollo SR, et al. 2010. J. Immunol. 184:5663.
3. Wang J, et al. 2010. P. Natl. Acad. Sci. USA. 107:1535.
4. Nieuwenhuis EE, et al. 2009. J. Clin. Invest. 119:1241. - Gene ID
- 25109 View all products for this Gene ID
- UniProt
- View information about CD1d on UniProt.org