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当前位置：首页 > 抗体蛋白 > 抗体> Brilliant Violet 421™ anti-mouse CD64 (Fc&ga

Brilliant Violet 421™ anti-mouse CD64 (Fc&ga

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke　AJ,　et al.　2020.　Proc Natl Acad Sci U S A.　117:33455-65. (SB)　PubMed
  6. Radtke　AJ,　et al.　2022.　Nat Protoc.　17:378-401. (SB)　PubMed
Product Citations
  1. Liu J, et al. 2017. Mucosal Immunol. 10.1038/mi.2016.139. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  4. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  5. Zhang H, et al. 2017. Leukemia. 10.1128/mBio.00226-17. PubMed
  6. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  7. Liu J, et al. 2020. Am J Pathol. 190:1298. PubMed
  8. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
  9. Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
  10. Kedage V, et al. 2022. MAbs. 14:2040083. PubMed
  11. Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed
  12. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  13. Schippers A, et al. 2015. Mucosal Immunol. 101038/mi201582. PubMed
  14. Matheis F, et al. 2020. Cell. 180(1):64-78.e16.. PubMed
  15. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  16. Lim HY et al. 2018. Immunity. 49(2):326-341 . PubMed
  17. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  18. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  19. Hiebert P et al. 2018. Developmental cell. 46(2):145-161 . PubMed
  20. Branchett WJ, et al. 2020. Wellcome Open Research. 0.278472222. PubMed
  21. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  22. Draheim M, et al. 2017. EMBO Mol Med. 1.489583333. PubMed
  23. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  24. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  25. Martinez-Turtos A, et al. 2022. Oncoimmunology. 11:2116844. PubMed
  26. Wang Y, et al. 2020. eLife. 9:e57438.. PubMed
  27. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  28. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  29. Liu J, et al. 2018. Am J Pathol. 188:2786. PubMed
  30. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  31. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  32. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  33. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  34. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  35. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
  36. Barnette DN, et al. 2018. JCI Insight. 3. PubMed
  37. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  38. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  39. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  40. Jones GS, et al. 2020. mSphere. 5:. PubMed
  41. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  42. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  43. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  44. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
RRID
AB_2562694 (BioLegend Cat. No. 139309)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
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