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Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- BALB/c mouse FcγRI-human IgG Fc fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.
- Application References
(PubMed link indicates BioLegend citation) -
- Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
- Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
- Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
- Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
- AB_2566558 (BioLegend Cat. No. 139319) AB_2566559 (BioLegend Cat. No. 139320)
Antigen Details
- Structure
- Ig superfamily, type I glycoprotein, 72 kD
- Distribution
-
Monocytes, macrophages, mast cells, dendritic cells
- Function
- Phagocytosis, ADCC
- Ligand/Receptor
- IgG
- Cell Type
- Dendritic cells, Macrophages, Mast cells, Monocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Gene ID
- 14129 View all products for this Gene ID
- UniProt
- View information about CD64 on UniProt.org
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