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Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E. coli-expressed, recombinant mouse IFN-γ
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
CyTOF? - Verified - Recommended Usage
This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.
- Application Notes
ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-? (Cat. No. 575309) as the standard. The LEAF? purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-?. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IFN-? bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-? in serum, plasma or supernatant, BioLegend's ELISA Max? Sets (Cat. No. 430801 to 430806) are specially developed and recommended.- Additional Product Notes
Maxpar? is a registered trademark of Standard BioTools Inc.
- Application References
(PubMed link indicates BioLegend citation) -
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
- Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
- Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
- Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
- Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
- Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
- Ferrick D, et al. 1995. Nature 373:255. (FC)
- Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
- Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
- DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
- Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
- Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
- Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
- Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
- Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
- Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
- Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
- Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
- Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
- Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
- Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
- Product Citations
-
- RRID
- AB_2562847 (BioLegend Cat. No. 505843)
Antigen Details
- Structure
- Cytokine; dimer; 40-80 kD (Mammalian)
- Bioactivity
- Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
- Cell Sources
- CD8+ and CD4+ T cells, NK cells
- Cell Targets
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- Receptors
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151. - Regulation
- Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
- Gene ID
- 15978 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org