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Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
- Application References
(PubMed link indicates BioLegend citation) -
- Doussis IA, et al. 1993. J. Clin. Pathol. 46:334.
- Davey FR, et al. 1988. J. Clin. Pathol. 41:753.
- Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
- Product Citations
-
- RRID
- AB_2074833 (BioLegend Cat. No. 333811) AB_2074832 (BioLegend Cat. No. 333812)
Antigen Details
- Structure
- Sialomucin family, 110 kD
- Distribution
-
Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Holness CL and Simmons DL. 1993. Blood 81:1607.
2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453.
3. Hameed A, et al. 1994. Hum. Pathol. 25:872. - Gene ID
- 968 View all products for this Gene ID
- UniProt
- View information about CD68 on UniProt.org
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