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Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine T cell lymphoma EL4.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone recognizes both alleles of ENPP1.
Additional reported applications (for the relevant formats) include: Immunoprecipitation.2- Application References
(PubMed link indicates BioLegend citation) -
- Wang H, et al. 2012. Proc Natl Acad Sci USA 109:20077. (FC)
- Abbasi S, et al. 2011. Hybridoma 30:11. (FC, IP)
- Waterfield JD and Fairhurst M. 1991. Immunology 72:226. (FC)
- Takei F. 1984. J. Immunol. 4:1951. (FC, IP)
- RRID
- AB_2564618 (BioLegend Cat. No. 149202)
Antigen Details
- Structure
- Type II transmembrane protein, 115 kD, and forms homodimers.
- Distribution
- Plasma cells, a subset of T cells, a subset of B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
- Function
- Generate pyrophosphate, recycle nucleotides, regulate cell adhesion, and to regulate signaling of insulin and purinergenic receptors.
- Cell Type
- Plasma cells, T cells, B cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Lau WM, et al. 2013. PLoS One. 8:e66752.
2. Kato K, et al. 2012. Proc. Natl. Acad. Sci. USA 109:16876.
3. Nam HK, et al. 2011. J Biol. Chem. 286:39059.
4. Zhou HH, et al. 2009. Eur. J. Pharmacol. 616:346. - Gene ID
- 18605 View all products for this Gene ID
- UniProt
- View information about ENPP1 on UniProt.org
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