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Biotin anti-mouse Ly-49A Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Con A activated splenic T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2

.

Application References

(PubMed link indicates BioLegend citation)
  1. Takei F. 1983. J. Immunol. 130:2794.
  2. Chan P, et al. 1989. J. Immunol. 142:1727.
Product Citations
  1. Pyzik M, et al. 2011. J Exp Med . 208:1105. PubMed
  2. Brodin P, et al. 2012. J Immunol. 188:2218. PubMed
  3. Brodin P, et al. 2010. PLoS One. 5:e13174. PubMed
RRID
AB_313754 (BioLegend Cat. No. 116803)

Antigen Details

Structure
C-type lectin, Ly-49, ITIM, 44 kD
Distribution

NK and NK-T cells

Function
Inhibitory NK cell receptor
Ligand/Receptor
MHC Class I Molecules
Cell Type
NK cells, NKT cells
Biology Area
Immunology
Antigen References

1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67.

Gene ID
16627 View all products for this Gene ID
UniProt
View information about Ly-49A on UniProt.org
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