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Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Con A activated splenic T cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2
.
- Application References
(PubMed link indicates BioLegend citation) -
- Takei F. 1983. J. Immunol. 130:2794.
- Chan P, et al. 1989. J. Immunol. 142:1727.
- Product Citations
-
- RRID
- AB_313754 (BioLegend Cat. No. 116803)
Antigen Details
- Structure
- C-type lectin, Ly-49, ITIM, 44 kD
- Distribution
-
NK and NK-T cells
- Function
- Inhibitory NK cell receptor
- Ligand/Receptor
- MHC Class I Molecules
- Cell Type
- NK cells, NKT cells
- Biology Area
- Immunology
- Antigen References
-
1. Coles MC, et al. 2000. Eur. J. Immunol. 30:236.
2. Takei FJ, et al. 1997. Immunol. Rev. 155:67.
3. Hanke T, et al. 1999. Immunity 11:67. - Gene ID
- 16627 View all products for this Gene ID
- UniProt
- View information about Ly-49A on UniProt.org