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PE anti-mouse TCR Vβ12 Antibody

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Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Joshi P, et al. 2006. J Leukoc Biol. 80:1345. (FC)
Product Citations
  1. Wang X, et al. 2022. Elife. 11:. PubMed
RRID
AB_10643999 (BioLegend Cat. No. 139703) AB_10639729 (BioLegend Cat. No. 139704)

Antigen Details

Structure
Member of the immunoglobulin superfamily, Vβ12 is a variant of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer
Distribution
subset of TCR αβ+ T cells
Function
Recognition of peptides presented in the MHC molecules, responsible for T cell mediated immune responses
Cell Type
T cells
Biology Area
Immunology, Adaptive Immunity
Molecular Family
TCRs
Gene ID
21577 View all products for this Gene ID
UniProt
View information about TCR V beta 12 on UniProt.org
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