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当前位置：首页 > 抗体蛋白 > 抗体> Brilliant Violet 510™ anti-human CD3 Antibod

Brilliant Violet 510™ anti-human CD3 Antibod

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Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex.

Clone OKT3 can block the binding of clones SK7 and UCHT1.4 The OKT3 antibody is able to induce T cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections and activation of T cells. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 317304). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 317326) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Li B, et al. 2005. Immunology 116:487.
  5. Jeong HY, et al. 2008. J. Leuckocyte Biol. 83:755. PubMed
  6. Alter G, et al. 2008. J. Virol. 82:9668. PubMed
  7. Manevich-Mendelson E, et al. 2009. Blood 114:2344. PubMed
  8. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
  9. Biggs MJ, et al. 2011. J. R. Soc. Interface. 8:1462. PubMed
Product Citations
  1. Wheatley A, et al. 2016. Sci Rep. 6: 26478. PubMed
  2. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  3. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  4. Ishizaka A, et al. 2016. J Virol. 90: 5665 - 5676. PubMed
  5. Andrews SF et al. 2019. Immunity. 51(2):398-410 . PubMed
  6. Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed
  7. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  8. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  9. Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
  10. Abreu C, et al. 2014. PLoS One. 9:97257. PubMed
  11. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  12. Piepenbrink MS, et al. 2021. Cell Reports Medicine. 2(3):100218. PubMed
  13. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  14. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
  15. Petrelli A, et al. 2018. J Clin Invest. 128:4669. PubMed
  16. Antonucci L, et al. 2020. Journal of Immunology Research. 3257.929861. PubMed
  17. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  18. Kreutmair S, et al. 2021. Immunity. . PubMed
  19. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  20. Tan J, et al. 2021. Sci Transl Med. 13: . PubMed
  21. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  22. Yu C, et al. 2021. Med (N Y). 2:755. PubMed
  23. Lu Q, et al. 2021. Immunity. 54(6):1304-1319.e9. PubMed
  24. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
  25. Sordé L, et al. 2017. Immunity, Inflammation, and Disease. 10.1002/iid3.167. PubMed
  26. van den Hoogen P, et al. 2020. PLoS One. 14:e0227283. PubMed
  27. Port JR, et al. 2020. J Virol. 94:. PubMed
  28. Einkauf KB, et al. 2019. J Clin Invest. 129:988. PubMed
  29. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
  30. Jiang C, et al. 2020. Nature. 261:585. PubMed
  31. Einkauf KB, et al. 2022. Cell. 185:266. PubMed
  32. Wang L, et al. 2021. Science. 373:. PubMed
  33. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  34. Gross C, et al. 2016. Proc Natl Acad Sci U S A. 113: 2973 - 2982. PubMed
  35. Dacon C, et al. 2022. Cell Host Microbe. Online ahead of print. PubMed
  36. Smalley M, et al. 2020. iScience. 23:101229. PubMed
  37. Hearps AC, et al. 2017. Front Immunol. 0.840972222. PubMed
  38. Cho H, et al. 2021. Sci Transl Med. 13:eabj5413. PubMed
  39. Cui J, et al. 2019. Neuro Oncol. 21:1436. PubMed
  40. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  41. Seydoux E, et al. 2020. Immunity. 53:98. PubMed
  42. Sant S, et al. 2020. PLoS Pathog. 16:e1008714. PubMed
  43. Burian A, et al. 2016. PLoS One. 11: 0163297. PubMed
  44. Aguilar–Brise? o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
  45. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID
AB_2561376 (BioLegend Cat. No. 317331) AB_2561943 (BioLegend Cat. No. 317332)

Antigen Details

Structure
Ig superfamily, the subunits CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) form the CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
  2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
  3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
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