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Concentration: 1mg/ml. Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Background: Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Purification : Purified by Protein-A affinity chromatography.
Application:
· Flow-Cyt: 1:20-1000
· Immunofluorescence: 1:20-1000
· Excitation spectrum: 649nm
· Emission spectrum: 670nm
· Optimal working dilutions must be determined by the end user.
· Protect from light.
Storage: Shipped at 4℃, Store at -20℃ (Avoid repeated freeze/thaw cycles).
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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