1、订购使用抗体产品的客户,在使用产品过程中遇到问题,提出技术支持及其他请求时,我公司接到请求后的24个小时之内做出处理。
2、我公司的所有产品都经过严格的质检后上架销售,如经复核确实存在问题,本公司无条件退款或更换货。
3、所有书面反馈我们收到后48小时内给出答复。
Ys10667R | |
英文名称 | Diphtheria toxin fragment A |
中文名称 | 白喉毒素A抗体 |
别 名 | DTX_CORBE; diphtheria toxin; NAD(+)--diphthamide ADP-ribosyltransferase (EC:2.4.2.36); EC:2.4.2.36; Diphtheria toxin fragment B; diphtheria toxin A; DTA; diphtheria toxin B; DTB; |
研究领域 | 细菌及病毒 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 21/62kDa |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Diphtheria toxin fragment A: 21-120/567 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 | Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B. Function: Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to eukaryotic elongation factor 2 (eEF-2). Fragment A is the catalytic portion responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A. Subcellular Location: Homodimer. SWISS: P10620 Gene ID: |
我要询价
*联系方式:
(可以是QQ、MSN、电子邮箱、电话等,您的联系方式不会被公开)
*内容: