文章标题：HNRNPF Increases MAF and CCNB2 Levels To Promote Cancer Cell Stemness and Progression of Pancreatic Ductal Adenocarcinoma
作者列表：Dong Shuchen, Teng Yue, Li Xingwang, Sun Xiaofeng, Zhu Huayun
期刊：Stem Cell Reviews and Reports
发表时间：2026-1-29
影响因子：4.2
DOI:10.1007/s12015-026-11066-z
主要研究成果：Abstract
Background
Tumor stemness, the capacity of a subset of cancer cells to self‑renew and drive tumor growth, has been implicated in pancreatic ductal adenocarcinoma (PDAC) aggressiveness. Heterogeneous nuclear ribonucleoprotein F (HNRNPF) is an RNA‑binding protein involved in mRNA processing, but its role in regulating stemness in PDAC remains unclear. This paper investigates the impact of HNRNPF on tumor stemness by regulating musculoaponeurotic fibrosarcoma (MAF) stability and cyclin B2 (CCNB2) transcription in PDAC.
Methods
The expression of HNRNPF, MAF, and CCNB2 in tumor and paracancerous tissues of PDAC patients was detected. The proliferative and invasive potential of PDAC was examined in vitro and in vivo after lentiviral intervention. A sphere formation assay was performed to analyze the self-renewal capacity of PDAC cells, and the expression of tumor stemness markers was detected. ChIP-qPCR and dual-luciferase assays were conducted to explore the transcriptional regulation between MAF and CCNB2. RNA immunoprecipitation and RNA decay assays were used to examine the regulation of HNRNPF on MAF.
