文章标题：Sulodexide improves vascular permeability via glycocalyx remodelling in endothelial cells during sepsis
作者列表：Jiayun Ying, Caiyan Zhang, Yaodong Wang, Tingyan Liu, Zhenhao Yu, Kexin Wang, Weiming Chen, Yufeng Zhou, Guoping Lu
发表时间：2023-8-8
期刊：Frontiers in Immunology
影响因子：7.3
DOI:10.3389/fimmu.2023.1172892
Abstract
Background
Degradation of the endothelial glycocalyx is critical for sepsis-associated lung injury and pulmonary vascular permeability. We investigated whether sulodexide, a precursor for the synthesis of glycosaminoglycans, plays a biological role in glycocalyx remodeling and improves endothelial barrier dysfunction in sepsis.
Methods
The number of children with septic shock that were admitted to the PICU at Children’s Hospital of Fudan University who enrolled in the study was 28. On days one and three after enrollment, venous blood samples were collected, and heparan sulfate, and syndecan-1 (SDC1) were assayed in the plasma. We established a cell model of glycocalyx shedding by heparinase III and induced sepsis in a mouse model via lipopolysaccharide (LPS) injection and cecal ligation and puncture (CLP). Sulodexide was administrated to prevent endothelial glycocalyx damage. Endothelial barrier function and expression of endothelial-related proteins were determined using permeability, western blot and immunofluorescent staining. The survival rate, histopathology evaluation of lungs and wet-to-dry lung weight ratio were also evaluated.
