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HRP标记的F(ab)2片段羊抗小鼠IgG H&L

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产品编号bs-60296G-HRP
英文名称F(ab')? Fragment Goat Anti-Mouse IgG H&L / HRP antibody
中文名称HRP标记的F(ab)2片段羊抗小鼠IgG H&L
别    名F(ab)2 Fragment Goat Anti-Mouse IgG H&L (HRP); F(ab)2 Fragment Goat Anti-Mouse IgG H&L/HRP; F(ab)2 Fragment Goat Anti-Mouse IgG(H+L);  
抗体来源Goat
克隆类型Polyclonal
交叉反应Mouse
产品应用IHC-P=1:1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状Liquid
浓    度1.0 mg/ml
免 疫 原Native Mouse IgG 
亚    型IgG
纯化方法affinity purified by Protein G
缓 冲 液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

产品图片
Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLP-1(1G9)) Monoclonal Antibody, Unconjugated (bsm-0933M) at 1:200 overnight at 4°C,An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37℃,DAB was used as the chromogen。
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (bsm-10832M) at 1:200 overnight at 4°C,An HRP-conjugated secondary (bs-60296G-HRP, 1:300 dilution) was used for 20min at 37℃,DAB was used as the chromogen。
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