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Cy5标记的羊抗小鼠IgG H&L

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产品编号bs-0296G-Cy5
英文名称Goat Anti-Mouse IgG H&L / Cy5 antibody
中文名称Cy5标记的羊抗小鼠IgG H&L
别    名Goat Anti-Mouse IgG H&L (Cy5); Immunoglobulin G;   
Specific References  (5)     |     bs-0296G-Cy5 has been referenced in 5 publications.
[IF=9.995] Huijuan Tan. et al. Phase separation of SGS3 drives siRNA body formation and promotes endogenous gene silencing. CELL REP. 2023 Jan;42:111985  IF ;  Arabidopsis seedlings,yeast.  
[IF=5.869]  Qianqian Guo . et al. Heterologous prime-boost immunization co-targeting dual antigens inhibit tumor growth and relapse. Oncoimmunology. 2020;9(1):1841392  ICC ;  Hamster.  
[IF=5.215] Tianrui Zhang. et al. Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization. PHARMACEUTICALS-BASE. 2023 Feb;16(2):243  FCM ;  Human.  
[IF=4.486] Shen L et al. FPS-ZM1 Alleviates Neuroinflammation in Focal Cerebral Ischemia Rats via Blocking Ligand/RAGE/DIAPH1 PathwayACS Chem Neurosci.2021 Jan 6;12(1):63-78.  IF ;  
[IF=4.021] Jiang X et al. Overexpression of augmenter of liver regeneration (ALR) mitigates the effect of H 2 O 2-induced endoplasmic reticulum stress in renal tubule epithelial cells. Apoptosis.2019.  ICF ;  Mouse.  
抗体来源Goat
克隆类型Polyclonal
交叉反应Mouse
产品应用IF=1:100-1000, Flow-Cyt=1:100-1000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性    状Liquid
浓    度2.0 mg/ml
免 疫 原Native Mouse IgG 
亚    型IgG
纯化方法affinity purified by Protein G, nonspecific adsorbed
缓 冲 液10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

产品图片
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 8) Monoclonal Antibody, Unconjugated (bsm-33061M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-cy5) for 90 minutes, and DAPI for nuclei staining.
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